
If you suspect you might be pregnant, one of the first questions that comes to mind is, “When should I take a pregnancy test?”
Getting the timing right is crucial to getting an accurate result, reducing stress, and making informed decisions about your next steps.
Whether you’re actively trying to conceive or concerned about an unplanned pregnancy, this guide will walk you through how pregnancy tests work, when and how to test, factors that affect accuracy, and what to do next.
How Pregnancy Tests Work
Pregnancy tests work by detecting the presence of human chorionic gonadotropin (hCG)—a hormone produced shortly after a fertilized egg attaches to the uterine lining.
After fertilization and implantation, hCG levels start to rise rapidly, doubling every 48 to 72 hours during the early weeks of pregnancy.
Most home pregnancy tests (HPTs) are designed to detect hCG in urine, while blood tests detect it in your bloodstream.
Understanding hCG is key to understanding why timing matters.
Too early = low hCG levels = possible false negatives.
When Is the Best Time to Take a Pregnancy Test?
Timing your test appropriately can save you unnecessary anxiety and confusion.

1. After a Missed Period
The gold standard for pregnancy testing is to wait until the first day after your missed period.
By then:
- hCG levels are typically high enough to be detected reliably.
- Most home pregnancy tests are 99% accurate at this point when used correctly.
- If you have regular menstrual cycles, this is the most reliable indicator.
2. Before a Missed Period
Some early detection pregnancy tests claim to identify pregnancy up to 6 days before your missed period.
However:
- Testing early often results in false negatives.
- Even if fertilization occurred, implantation and sufficient hCG production may not have happened yet.
- If you choose to test early and get a negative result, retest after your missed period for confirmation.
What Is the Best Time of Day to Take a Pregnancy Test?
Morning is best.
First-morning urine tends to be the most concentrated, making it more likely that even low levels of hCG will be detected.
Drinking lots of water before testing can result in diluted urine and lead to inaccurate results.
If testing later in the day, try to avoid drinking large amounts of fluids for at least two hours before taking the test.

Factors That Affect Pregnancy Test Accuracy
Several things can impact whether your pregnancy test gives you an accurate result:
1. Timing
Testing too soon—even with early detection tests—can produce false negatives.
2. Test Sensitivity
Not all pregnancy tests are created equal.
- Highly sensitive tests can detect lower levels of hCG (as low as 6.3 mIU/mL).
- Others require higher hCG levels to produce a positive result.
- Read packaging carefully when selecting a test.
3. Urine Dilution
Over-hydrating before taking a test can dilute hCG levels, leading to a false negative.
4. Improper Use
Common user errors include:
- Not following the test instructions carefully.
- Not waiting the correct amount of time to read the result.
- Reading the result window too late, causing evaporation lines to be mistaken for a positive.
5. Medications
Certain medications—like fertility drugs containing hCG—can influence pregnancy test results.
Other medications (such as antibiotics, birth control, or pain relievers) usually do not affect results.
Understanding Pregnancy Test Results
False Negatives
Reasons you might get a false negative:
- Testing too early.
- Using diluted urine.
- Faulty test or expired product.
False Positives
Rare, but possible. Reasons include:
- Recent miscarriage or abortion (hCG may linger).
- Certain rare medical conditions (e.g., ovarian cysts or some cancers).
- Use of fertility treatments containing hCG.
Always confirm a positive home test with a healthcare provider.
Types of Pregnancy Tests
1. Urine Tests
Home pregnancy tests detect hCG in urine:
- Widely available over the counter.
- Quick results (typically within 3–5 minutes).
- Affordable and convenient.
2. Blood Tests
Conducted at clinics or doctor’s offices:
- Can detect pregnancy earlier (as soon as 6–8 days after fertilization).
- Measure the exact amount of hCG in your blood.
- Useful in monitoring pregnancy health (especially with concerns like ectopic pregnancy or miscarriage).
Early Signs That Might Prompt Testing
Before your missed period, you might experience early pregnancy symptoms such as:
- Implantation bleeding (light spotting 6–12 days after ovulation)
- Breast tenderness
- Nausea or food aversions
- Fatigue
- Mood swings
- Frequent urination
- Heightened sense of smell
- Mild cramping
Note: These symptoms can also mimic PMS, so a pregnancy test is necessary for confirmation.
How to Take a Pregnancy Test Correctly
To maximize accuracy:
- Read instructions carefully before starting.
- Use first-morning urine if possible.
- Avoid over-hydration before testing.
- Time the test properly—follow the manufacturer’s recommended waiting time.
- Interpret results within the suggested time window.
What to Do After Taking a Pregnancy Test
If the Test Is Positive
- Confirm with a healthcare provider.
- Schedule an ultrasound to determine gestational age and viability.
- Start considering your options—parenting, adoption, or abortion.
- Free clinics like Ava Health offer confidential services to help you navigate the next steps.
If the Test Is Negative
- If your period still hasn’t arrived after 3–5 days, retest.
- Consult a healthcare provider if cycles remain irregular or symptoms persist.
Common Myths About Pregnancy Testing
Myth 1: Testing any time after sex will tell you if you’re pregnant.
Reality: hCG only begins production after implantation—several days after fertilization.
Myth 2: A negative result always means you’re not pregnant.
Reality: Early testing can miss low hCG levels. Always retest or consult a healthcare provider if unsure.
Myth 3: Drinking lots of water before testing improves accuracy.
Reality: Too much fluid dilutes urine and can actually decrease the accuracy of your test.
Where to Get Help and Support
Facing uncertainty about pregnancy can be emotionally taxing.
Ava Health in Portland offers free, compassionate, confidential support, including:
- Lab-grade urine-based pregnancy testing
- Limited obstetric ultrasounds
- Comprehensive Medical Education about your pregnancy options
- Referrals for prenatal care, adoption services, or abortion education
Contact Ava Health:
📞 Call: (971) 358-3797
🌐 Visit: avahealthpdx.org
Conclusion: Timing Is Everything
If you think you might be pregnant, timing your test carefully will give you the most accurate result and the peace of mind you deserve.
- Wait until at least the first day after your missed period.
- Use first-morning urine.
- Follow instructions closely.
- Confirm positive results with a healthcare provider.
No matter what you’re feeling right now—hope, fear, excitement, anxiety—you don’t have to navigate this moment alone.
Ava Health is here to provide clarity and compassion every step of the way.
Sources
-
American College of Obstetricians and Gynecologists (ACOG).
“Early Pregnancy Loss.” ACOG, Dec. 2020, www.acog.org/womens-health/faqs/early-pregnancy-loss. -
Mayo Clinic Staff.
“Home pregnancy tests: Can you trust the results?” Mayo Clinic, https://www.mayoclinic.org/healthy-lifestyle/getting-pregnant/in-depth/home-pregnancy-tests/art-20047940 -
American Pregnancy Association.
“Am I Pregnant.” American Pregnancy Association, 2024, https://americanpregnancy.org/getting-pregnant/am-i-pregnant/ -
U.S. Food and Drug Administration (FDA).
“Home Use Tests: Pregnancy.” FDA, 23 Oct. 2020, www.fda.gov/medical-devices/home-use-tests/pregnancy.