How Pregnancy Tests Work: Everything You Need to Know 

Pregnancy is a life-changing event. Knowing whether or not you are pregnant as early as possible is crucial for making informed decisions about your health and future. Pregnancy testing is the first essential step toward confirming a pregnancy. While a positive result on an at-home test is often reliable, it’s important to follow up with a medical professional to verify the pregnancy and gather critical information about your health. 

At Ava Health, we offer no-cost, confidential services designed to provide you with trustworthy answers and compassionate care during what can be an overwhelming time. In this guide, we’ll walk you through how pregnancy tests work, the importance of accurate testing, when and how to test, and what your next steps should be after a positive result. 

What Is a Pregnancy Test?

A pregnancy test is a method used to detect the presence of a specific hormone in your body called hCG (human chorionic gonadotropin). This hormone is only produced during pregnancy, beginning when a fertilized egg attaches to the lining of the uterus. 

Pregnancy tests come in two primary forms: 

  • Urine Tests: These are available over the counter for home use or can be administered at a clinic. 
  • Blood Tests: These are performed at medical facilities and can detect pregnancy earlier than urine tests. 

Regardless of the method, the goal is the same: to determine if hCG is present in your body. 

How Urine Pregnancy Tests Work

Most people are familiar with an at-home urine pregnancy test. They are simple, affordable, and convenient. Here’s how they work: 

  • You collect a small sample of your urine and expose it to a test strip. 
  • The strip contains antibodies designed to react with hCG if it is present. 
  • If hCG is detected, the test will show a positive result, often indicated by lines, plus signs, or digital words like “pregnant.” 

Important Tip: For the most accurate result, it’s best to test first thing in the morning when your urine is most concentrated with hCG. 

At-home pregnancy test with positive result shown on the display.

How Blood Pregnancy Tests Work

Blood-based pregnancy tests are more sensitive and can detect lower levels of hCG earlier in pregnancy—sometimes as soon as 6–8 days after ovulation. 

There are two types of blood pregnancy tests: 

  • Qualitative hCG Test: Simply detects if hCG is present (positive or negative). 
  • Quantitative hCG Test (Beta hCG): Measures the exact amount of hCG, which can help determine how far along you are and monitor for potential issues. 

Blood tests are typically used in clinical settings when early pregnancy confirmation is critical or when a more detailed measurement is needed. 

When Should You Take a Pregnancy Test?

Timing is everything when it comes to pregnancy testing. Taking a test too early can lead to inaccurate results. Here’s what you should know: 

  • After a Missed Period: Most at-home tests claim to be 99% accurate when taken after you miss your period. 
  • Early Testing: Some sensitive tests claim to detect pregnancy days before a missed period, but early testing increases the risk of a false-negative. 
  • Best Practices: If possible, wait at least a few days after your missed period to ensure hCG levels are high enough for detection. 

Key Tip: If your first test is negative but you still suspect you’re pregnant, retest after a few days. 

Can You Get a False Positive or False Negative?

While pregnancy tests are highly accurate when used correctly, false results can occur. 

False-Negative Results 

A false-negative result means the test says you’re not pregnant when you actually are. Reasons include: 

  • Testing too early 
  • Diluted urine (from drinking too much fluid) 
  • Expired or defective test kit 
  • Not following test instructions correctly 

False-Positive Results 

False positives are less common but can happen due to: 

  • Recent miscarriage or abortion 
  • Certain medications containing hCG 
  • Medical conditions such as rare ovarian tumors 

Because of the possibility of false results, it’s important to confirm any positive test with a medical professional. 

Comparison chart of blood pregnancy test vs urine pregnancy test features

Why Medical-Grade Pregnancy Testing Matters

While over-the-counter tests are a great first step, medical-grade pregnancy testing provides the highest level of accuracy. Clinics like Ava Health offer professional testing to ensure your results are trustworthy. 

Medical-grade tests are especially important because they: 

  • Use higher sensitivity equipment 
  • Offer expert interpretation by trained medical professionals 
  • Reduce the likelihood of error 
  • Provide the opportunity for additional services like ultrasounds 

What Happens After a Positive Pregnancy Test?

If your pregnancy test is positive, the next step is to confirm the pregnancy with an ultrasound. An ultrasound can provide critical information such as: 

  • Viability: Is the pregnancy developing normally with a detectable heartbeat? 
  • Gestational Age: How far along are you? Knowing this helps in making informed decisions. 
  • Location: Is the pregnancy located inside the uterus? (An ectopic pregnancy, which occurs outside the uterus, can be life-threatening.) 

At Ava Health, if you receive a positive pregnancy test with us, you may qualify for a no-cost limited obstetric ultrasound to get these vital answers. 

Factors That Affect Pregnancy Testing Accuracy

Several factors can impact the accuracy of your pregnancy test: 

  • Timing: Testing too soon may not give your body enough time to produce detectable levels of hCG. 
  • User Error: Not following the instructions exactly can skew results. 
  • Test Sensitivity: Different brands have different sensitivity levels to hCG. 
  • Medications: Fertility treatments containing hCG can lead to false positives. 
  • Health Conditions: Rare medical conditions may produce hCG without a pregnancy. 

Common Early Pregnancy Symptoms

While pregnancy tests are the gold standard for confirmation, many women experience early signs of pregnancy that might prompt them to test: 

  • Missed period 
  • Nausea or vomiting 
  • Frequent urination 
  • Fatigue 
  • Breast tenderness 
  • Mood swings 
  • Food aversions or cravings 

If you experience these symptoms, it might be time to take a pregnancy test. 

FAQs About Pregnancy Testing

How Soon Can I Take a Pregnancy Test After Sex? 

Typically, you need to wait about 2–3 weeks after having unprotected sex before testing. Testing earlier may yield unreliable results. 

What if I Have Irregular Periods? 

If your periods are irregular, it can be harder to know when to test. In general, waiting about three weeks after sexual intercourse can help improve accuracy. 

Can Medications Affect Pregnancy Test Results? 

Some fertility medications contain hCG and can cause false positives. Most medications, including birth control pills and antibiotics, do not affect test results. 

Do I Need a Blood Test if I Have a Positive Urine Test? 

In most cases, a positive urine test followed by an ultrasound is sufficient. However, your healthcare provider may order a blood test in specific situations. 

How Ava Health Can Support You

At Ava Health, we’re here to provide more than just answers—we offer compassionate, judgment-free support. We understand the emotional weight that can come with a potential pregnancy, and we are here to walk beside you every step of the way. 

Our services include: 

  • No-cost, medical-grade pregnancy testing 
  • Limited first or second-trimester obstetric ultrasounds 
  • Comprehensive options education 
  • Emotional and practical support 

We believe every woman deserves to feel empowered and informed about her health. 

You are not alone.

Schedule Your No-Cost Pregnancy Test Today

If you think you might be pregnant, don’t wait. Contact Ava Health today to schedule your confidential appointment with our licensed medical team. Whether you’re seeking confirmation of a home test or are looking for next steps, we are here to help you with care, compassion, and expertise. 

Call us today or book your appointment online. 

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